SINBIONESA: Synbiotic Powder Made of Indigenous Microorganisms from Goat Digestive Tract with Rice Bran and Corn Flour Fillers

Abstract

This study aims to evaluate the quality of SINBIONESA produced to meet the probiotic quality of the SNI benchmark. Identifying potential probiotic bacteria isolated from a goat's digestive tract by using molecular analysis of the 16S rRNA gene was conducted. Selected isolates were observed and tested to identify potential bacteria as probiotics, including cell shape, gram methods, positive character test, negative character test, cellulolytic activity test, optimal pH, optimum temperature, acid resistance, and resistance to bile salts. The selected bacteria were formulated with a filler of 75% rice bran and 25% corn starch, then dried by freeze-drying. SINBIONESA quality was evaluated on the moisture content (by drying technique), the total number of probiotics (by total plate count), and the expiration period (linear regression formula). The results show that SINBIONESA has three potential probiotic bacteria: Bacillus pumilus, Bacillus brevis, and Pseudomonas diminuta. It has a water content of 2.89% with an expiration period at room temperature storage and cold temperatures of 1 year, four months, and two years five months, respectively. Based on these results, it is stated that SINBIONESA has met the SNI quality of probiotics in Indonesia.